Nora Clavicle, a women's rights activist, uses her influence to have Commissioner Gordon, Chief O'Hara, and the whole police force fired and replaced with women. She cuts ties with Batman and Robin, then launches her plan to blow up Gotham City with explosive-packed mice and collect on the insurance. The Terrific Trio look for a way to neutralize her mechanical hordes before it's too late.

Colonel Gumm moves out of the Pink Chip Stamps Factory, taking its owner Pinky Pinkston along as a hostage. A message hidden by Miss Pinkston in a bowl of alphabet soup directs Batman and Robin to the International Stamp Exhibition. Though the Green Hornet and Kato will be attending to try to expose Colonel Gumm, Batman and Robin believe they are out for personal profit. The misunderstanding has the two sets of crime fighters fighting each other while the real criminal nearly makes off with the exhibition's rare and valuable stamps.

The Black Widow uses her cerebrum short-circuiting device to rob bank after bank in Gotham City. Batman and Robin soon catch up with her, but actually capturing the villainess proves harder than expected. Despite her nerve paralyzer spray, and two remote-control dummies that try to throw the Dynamic Duo off course, they finally make it inside the Black Widow's lair. But soon after entering, they are trapped in a giant web with two deadly black widow spiders.

Louie the Lilac is in town, planning to corner the lilac perfume market in Gotham City. His concoction requires the glands of several animals, so he kidnaps Bruce Wayne and Dick Grayson to make the extractions. With "Batman and Robin" unable to respond, Batgirl attempts a rescue, only to be captured herself. The trio rely on their wits (and the latest bat-invention) to attempt an escape.

Archer and his gang have come to Gotham City, winning the public's favor with their Robin Hood style deeds of stealing from the rich to give to the poor. Batman and Robin set out to discover the location of their hideout and stop these villains, only to find themselves caught by a trap in the Archer's lair.

The Black Widow adjusts her cerebrum short-circuiting device to successfully alter Batman's brain. With Batman powerless to resist, and Robin captured, she disguises herself as the Boy Wonder and brings along a dummy Batman to rob another bank. Commissioner Gordon initially believes Batman is setting a trap for the villainess. But when word comes of the successful robbery, the commissioner puts out an all-points bulletin to bring in the Caped Crusaders dead or alive.

Batman and Robin escape their previous peril and are back in action seeking to foil the Archer and his gang. After pulling off a counterfeit job, the villains head for their boat, planning to take their loot safely to Switzerland. The Dynamic Duo set off in the Bat boat to try to stop their escape.

The Puzzler continues with his plan to hijack Artemus Knab's new airplane, the Retsoor, all the while leaving puzzles behind for Batman and Robin to ponder. He familiarizes himself with the jet's designs, then prepares to fly his prize to a foreign country where he will hold it for ransom. But the Dynamic Duo have an easier time with his clues than he expected, resulting in a climactic confrontation at the airplane hanger.

The Penguin teams up with parasol collector Lola Lasagne. Together, they plan to rig the upcoming Bruce Wayne Handicap to make a fortune from Lola's prize racehorse Parasol. They break into Glu Gluten's Glue Factory to steal a condemned horse, planning to switch horses before the race and make their money by betting on the real Parasol. When Batman, Robin, and Batgirl catch up with them at the glue factory, Penguin sneaks outside and plasters the Batmobile with a sticky glue.

The Puzzler, a villain with a fondness for both Shakespeare and aviation, indicates he is after the fortunes of Artemus Knab. He convinces the billionaire to invest in his puzzle balloon business, but when the Dynamic Duo find that Knab seems too intelligent to fall for a phony scheme, they look deeper for the criminal's true intentions. Their search leads them to Knab's airplane monopoly, but unknown to them, the Puzzler has plans to halt their investigation - permanently.

Minerva has been using her "Deepest Secret Extractor," disguised as a piece of equipment at her mineral spa, to discover where her wealthiest customers hide their valuables. With this info in hand, Minerva has been able to pull off a string of robberies with ease. When Batman and Robin's investigation is cut short by the villainess, butler Alfred goes undercover to help foil her criminal operations.

The Dynamic Duo arrange a trap for an elusive annual bank robber, but the female magician they are hunting is on to them with a new scheme of her own.

Told that her loot is genuine money after all, Zelda is forced to lure Batman and Robin into a possibly unsolvable deathtrap, with hitmen waiting outside to shoot them if they escape.

Though Batman escapes his watery trap, Robin is left in the hands of King Tut. The deluded villain still believes that Lisa Carson is really Queen Cleopatra, and calls Lisa's father to demand a ransom of $8,300,487.12, the mortgage on the Pyramids. Using the Jolly Jackson radio show to communicate, Tut negotiates the terms of payment with Batman. However, the villain most likely intends to keep both the money and Lisa, and has further plans to fry the Caped Crusaders in his royal boiling oil.

The Joker appears in an art gallery and defaces the paintings with paint-spraying guns. But instead of being horrified at his alterations, the Joker's work is praised as "art". After more success at an international art competition, the Joker founds his own art school. But Batman and Robin suspect he has a more sinister purpose in mind when he enrolls only wealthy millionaires.

Mr. Freeze is back in town, and has plans to build an icy weapon that will have the world at his control. With help from Glacia Glaze, the renowned ice skater, he captures Professor Isaacson and tries to extract the secret formula for instant ice from him. Mr. Freeze then sends his trained seal Isolde to Police Headquarters with a note, demanding Batman deliver a ransom in exchange for the professor. But the frosty villain not only plans to keep the money and the professor, but to terminate the Dynamic Duo in the process.

Back in his iceberg headquarters, Mr. Freeze persuades the kidnapped Professor Isaacson to build him a thermodynamic ice ray beam. He issues an ultimatum that he will begin freezing the entire country if his demands are not met, then gives a demonstration of his weapon. As the villain works out just what his demands are, Batman and Robin track his seal, Isolde, through an ice-packed Gotham Harbor to locate the villain's hideout.

Louie the Lilac has cornered the flower market in Gotham City, hoping to gain control over the entire "flower generation." He kidnaps the organizer of their planned flower-in, Princess Primrose, and brainwashes her with his Stupefying Aromatic Spray. Batman and Robin follow a clue to the villain's lair only to be captured by Louie and fed to his giant man-eating lilacs. With the Caped Crusaders out of the way, Louie heads back to the flower-in to continue his evil plan. Alfred, meanwhile, has been alerted to Batman and Robin's situation, and goes looking for Batgirl to aid in the fight.

Egghead and Olga, Queen of the Cossacks, steal two pounds of Radium from the Gotham City Radium Center. Using it in their "Zapping Machine," they attempt to hatch an ancient Neosaurus egg, planning to feed the baby dinosaur a meal of Batman, Robin, and Batgirl. Surprisingly, the machine seems to be working. But when the egg cracks, the baby Neosaurus is more than they can handle.

Lord Ffogg and Lady Peasoup plan to steal a ship-worth of mod clothing material with which they can control the fashion world of Londinium. Ffogg and his men subdue Batman, Peasoup and her girls grab Robin and Lady Prudence uses the paralyzing fog on Batgirl.

Batman, ably assisted as always by Alfred, saves Robin from being crushed by a winch at the Tower of Londinium. He also rescues Batgirl from the dungeon at Ffog Place. But Robin gets stung by an African Death Bee as Lord Ffogg and Lady Peasoup get ready to steal the crown jewels at the Bloody Tower.

The Catwoman targets the fashion industry by first terrorizing a banquet honoring Batgirl and then attacking a fashion show. During the attack on the fashion show, Catwoman captures Batgirl and takes her back to her hideout where she threatens to kill her with a pattern cutter. She then tells Batman that if he attempts to rescue her that it will leave the visiting Queen Bess of Bellgravia vulnerable for attack. Now the Caped Crusader must find a way to save Batgirl and prevent any harm to Queen Bess.

Egghead and Olga, Queen of the Cossacks, kick off their return with several thefts from the Gotham City Museum. Egghead begins planning a series of heists - intended to provide him with the dowry needed to marry Olga. Swiping 500 pounds of dehydrated caviar goes off without a hitch. But when Batgirl attempts to outfox Egghead, she is instead captured by the villain. It's up to Batman and Robin to find the villains' hideout before Olga's Cossacks make mincemeat out of their crime fighting friend.

Following a tip from Batgirl, the Dynamic Trio meet up at the Grimalkin Novelty Company, hideout of the Joker and Catwoman. There, they overhear the arch-criminals' plot and secretly trail them to a stash of hidden gunpowder. A careless mistake spoils the villains' evil plan, however, and they are captured and brought before the Gotham City judge. But when the jury seems less than interested in justice, courtroom mayhem ensues.

The Penguin taints the ink at the U.S. Mint with Lygerian Sleeping Sickness. As word of the infected money gets out, citizens of Gotham City empty their cash right onto the streets - and into Penguin's pockets. But when Bruce Wayne spreads word of the infected bills worldwide, the Penguin realizes his ill-gotten gain is worthless and threatens a more devilish plot if the warning is not retracted.