A meeting on the Assassin’s Road causes Itto to remember the events leading up to his wife’s murder, and why he chose to bring his son with him on his mission of vengeance.

Multiple stories interweave as Daigoro becomes lost while seeking medicine for his father and is befriended by a young woman who is being sold into service by a loan shark.

When his father is delayed for multiple days after an assassination goes awry, a desperate Daigoro wanders off and falls into the hands of a ruthless gang of criminals.

Itto accepts an assignment to stop a shipment of gold, assassinate a government official, kill an entire team of covert agents and prevent a small clan from being framed.

As Lord Yagyu prepares to send his last and strongest son, Gunbei, to kill Itto Ogami, the events of Gunbei and Itto's previous duel are revealed.

A scheming magistrate's plan places Itto in the middle of a war between two Yakuza families, one of which is lead by the daughter of the leader of the other.

While the Yagyu set a trap for Itto, a group of con artists pose as Lone Wolf and Cub and a disgraced Samurai takes on the role of Ogami.

As Itto waits for an opportunity to assassinate a sadistic Lord, he and Daigoro observe the life of a young boat woman who must sell herself to support the man she loves.

While Itto tracks down the killers of a young woman's entire family, Daigoro becomes caught up in a policeman's hunt for a notorious female pickpocket.

Itto accepts a job that will protect the location of a secret gold mine, but the task is complicated when a rival swordsman takes Itto's target's son hostage.

Itto must deal with a well-wishing doctor and a group of shadow ninja when he's tasked with killing a group of assassins who're rgeting the Shogun's lover.

Itto returns to a spot on the banks of the Shinobu River where he once executed the lord of a Clan, and faces a samurai he defeated in a duel four years earlier.

Government officials convince Asaemon the Beheader, one of the few swordsmen in Japan with a level of skill close to Ogami’s, to kill Itto in combat.

Itto meets Sakon Shino, a beggar samurai who has given up killing for a life as a street performer. Shino urges Itto to give up his assassin life, for the sake of his son.

The Lone Wolf and Cub have their work cut out for them when a seemingly simple job turns into a deadly game of lies and intrigue.

The Lone Wolf and Cub rescue a woman who has sold herself into prostitution in order to stage her revenge on her murdered brother’s evil fiancé.

Desperate to keep their clan from being dissolved, the family of a murdered Lord fake the real killer’s murder and ask Itto to verify their false claim.

Assassins from the Kurokuwa clan stage a non-stop series of attacks that lasts for days, slowly steering an exhausted Lone Wolf and Cub into a deadly trap.

Itto is hired to kill a samurai who has allegedly turned his men against Lord Norikatsu, but the true reasons turn out to be far more complex and deceptive.

Ogami mysteriously joins a group of “the unclothed”, men with the unusual job of carrying people and baggage across a river where bridges and ferries are forbidden.

After being thrown off a cliff during a battle with the Yagyu assassins, Daigoro searches for his now missing father while avoiding his family’s many enemies.

Itto tries to follow the trail left by his missing son, Daigoro, while fighting off the forces of Lord Retsudo and his Yagyu assassins.

In honor of a woman who saved his son’s life, Itto goes to assist a group of farmers who have been conscripted by the ruthless Lord Ichige and his brother.

With no warriors left who are capable of beating Itto physically, the Yagyu resort to coercing a ninja to use the secret five prong technique against the Lone Wolf.

Itto is hired by a Clan Lord to kill a spy who seduced and impregnated his daughter, while Daigoro befriends the woman’s now four year old son.