Rin enters his "flow," and Isagi's faith in Rin's trance state pays off when Isagi applies what he's learned about the mechanism of luck back when he lost to Rin. Can the Blue Lock XI beat Japan's U-20s, and what awaits them after the match?

Seeing the players' upgrade, Sae takes the play to the next level. In response, Rin starts to align his thought process to Sae and drastically changes his play style . Then Rin realizes that Blue Lock players have been keeping up with his play.

35 forwards remain after the 2nd selection at Blue Lock. With a big match against Japan's U-20s in three weeks, Ego announces that the team will be centered around the top 6 players. The tryouts for the rest of the positions begin.

Isagi relies on "the smell of the goal," his raw instincts, to make a move, and it leads to the fierce battle of "reading" each other's hand with Team Y's playmaker, Niko Ikki. Isagi and Niko's battle as they both watch over the whole field and throw their ego at each other, brings them to the last one minute of the match. Can Isagi and Team Z beat Niko's final strategy, "One Time Kill Counter"?

Team Z was crushed by Team X, who centered the offensive play around egoistical yet skilled Barou. Responding to Ego's urge to "take up a weapon," the players discuss what their weapons are in preparation for the next match. However, Isagi struggles to identify what it is. Using "the smell of the goal" he felt in the game against Team X as a hint, Isagi and the others go against Team Y in the next match.

Isagi Yoichi, a first-year forward, loses his chance for the Nationals because he has passed the ball to his fellow player in front of the goal. That's when he receives an invitation for Blue Lock. It's a facility to train one world's greatest striker out of 300 high-school-aged forwards so that Japan can win the World Cup.

Kira is eliminated from Blue Lock, and with that, the first day at the Blue Lock that promises to create the greatest center forward in the world begins in Isagi Yoichi 's life.

The first selection is the round-robin tournament of five teams of V, W, X, Y and Z in Building Five, with only top two teams being eligible to move forward. Isagi's Team Z, the lowest ranking team, plays against Team X. But because of the special rule that allows the top scorer of the team to proceed to the next section, the match turns into chaotic scrambling for the ball, regardless of the teams. It was Barou Shoei, Team X's formidable striker, who changes the flow of the game.

After the match with Team Y, Isagi's rank moved up, and he became the center of the team. The next match is against Team W, led by the Wanima twins. Their eye contact and impeccable combinations are hard to beat. They used to play in the same team as Chigiri Hyoma, and they use his past trauma to egg him on. Chigiri says that he joined Blue Lock to find a reason to quit soccer. Team Z leads the game with Kuon's play. Everyone's elated for the good start of the match, but Isagi feels something is off.

Finally, Isagi and Nagi are going to have a rematch with Rin's team. However, adding Bachira to Rin's team will bring some unforeseen circumstances.

Because of Kuon’s betrayal, Team Z is faced with a desperate situation of playing 12-on-10, with the opponent knowing everything about their weapons and strategies. Even under such a circumstance, Team Z never gives up on the fight, and Isagi continues to believe in himself. Inspired by their passion, Chigiri runs through the field at an incredible speed, his weapon he has sealed away. His ego rocks the match when they are losing at 3-4.

When Itoshi Sae scores the first goal of the match, the crowd goes wild, making it clear to Team Blue Lock that they're here to watch Sae. Rin changes the strategy and Nagi makes the memorable first goal for Team Blue Lock.

Shidou joins the match in the second half and throws everyone off with his crazy play. After he scores a free kick, Chigiri and Niko get swapped out with Reo and Hiori. With Blue Lock’s new formation, Reo proves his worth with Chameleon Defense.

Shidou enters the "flow" and unleashes his Big Bang Drive to score another goal. Isagi demands that Ego let them win, and Ego subs Barou in to cause chaos on the field. And the other players start to find their "flow"...

As Aiku looks back on why he converted to a defender, the U-20s uses their specialty quick attack. Reo copies Nagi's skill to hold them off. Isagi yields the last step to Rin, which is stolen by Barou who has waited for the chance in the dark.

The Z team has only one game left to play, and it will decide the future of its members. While everyone seems to be nervous about the kickoff, Isagi is trying to find a way to score goals without anyone's help.

Each player unleashed their weapon and ego, and Team Z has caught up to a draw. They totally have the momentum of the game, but that has “awakened” Team V’s monster, Nagi. In the chain reaction to awakening, Isagi also learns to use his weapon and keeps fighting. The “heat” generated by the clashing of ego from both teams starts to affect Kuon as well.

Team Z launches a final counter to try and win against Team V. Isagi is trying to find the last piece he needs to crack the formula to become a goal machine.

With many dropouts, the "Blue Lock" project enters the second selection. A harsh second selection where former friends become rivals and former enemies become allies at a dizzying pace. There, Isagi meets Rin, the most powerful egoist ever, the younger brother of the genius Itoshi Sae, and the new Blue Lock ranking TOP ranker. Seeking further evolution, Isagi's fight advances to a new stage.

Seeing how incompatible Rin and Shidou are, Isagi hopes to take advantage of it. However, his physical abilities are not enough to get him where he needs to be. Then, Hiori suggests that Isagi does the thinking "on reflex."

The match continues to leave Team Z scared of Nagi's abilities. However, one of the members is not scared, even though they are facing a soccer genius...?

By understanding and enhancing each other’s weapons, Isagi and Nagi are drawing countless paths to the goal. In contrast, with Chigiri, who has further evolved, at the center, Kunigami and Reo find themselves struggling to keep up, and a back-and-forth battle unfolds on the field. Meanwhile, Barou, who should have been the king of the field, was left behind in the evolutionary chain because of his overpowering ego. Having been robbed of the leading role on the field by Isagi, Barou finally falls to his knees.

In this new stage of the second selection, the white team formed by Isagi, Nagi and Bachira will finally go against the top three in the ranking of the previous stage, running the risk of losing one of their three players if they lose the game. Still, the willingness to test their new skills against the top three is much stronger in the white team members. Will they be able to win this match?

Rin chooses to add Isagi to his team, and together with Bachira they pass the second selection. Waiting for them on the other side is a 5-on-5 match against a team of world-class players. Thanks to Isagi, Bachira, and Rin's coordinated play, they manage to score the first goal, but they soon find themselves overwhelmed by the opposing team's superior skill and ability. Despite this, they desperately keep fighting until the last second, unleashing their own "egos." As a result, the "Blue Lock" project, aiming to produce the world's greatest striker, enters a new phase.

Isagi is chosen as Team A1 along with Nanase and Hiori to play against Team B1, which includes Karasu, Otoya, and Chigiri. While Isagi struggles to find a way into the game, Chigiri and Hiori demonstrate their worth.