The tryouts are complete, and Isagi is chosen for the starting lineup. But Shidou is nowhere to be seen... It turns out Sae has picked him to join the U-20s. Meanwhile, Ego begins the final training camp, where he discusses the secret of "flow."

The exhibition match between Blue Lock and Japan's U-20s has finally kicked off. The first play Bachira and Isagi set up has been swiftly shut down by the U-20s' captain, Aiku, and Isagi finds a new challenge in him.

Isagi is chosen as Team A1 along with Nanase and Hiori to play against Team B1, which includes Karasu, Otoya, and Chigiri. While Isagi struggles to find a way into the game, Chigiri and Hiori demonstrate their worth.

Isagi looks back and analyzes what he did when he scored a goal on reflex. Then, when the second match begins, Reo finds his new playstyle: Chameleon.

Rin and Sae's sibling match goes to Sae as he takes the ball. But Niko, Karasu, Otoya, and Chigiri set up another goal chance, and Rin scores despite shooting on his non-dominant leg. At 1-2, the U-20s are finally ready to go all out.

Isagi, Bachira, and Chigiri's families are happy to see their sons on the field. Meanwhile, we get a glimpse of what transpired between Rin and Sae. Sae is about to quit the match, but he decides to stay when Shidou is thrown into the fray.

Seeing how incompatible Rin and Shidou are, Isagi hopes to take advantage of it. However, his physical abilities are not enough to get him where he needs to be. Then, Hiori suggests that Isagi does the thinking "on reflex."

Kira is eliminated from Blue Lock, and with that, the first day at the Blue Lock that promises to create the greatest center forward in the world begins in Isagi Yoichi 's life.

The loss of Bachira on his team makes Isagi very anxious about what his future with Nagi will be like. To make matters worse, the situation is now even more complex: the ideal would be to go against a weaker team, but that would mean taking a weaker player too, which would make it more difficult to advance the next stages. So what are Isagi and Nagi going to do?

Barou joins Isagi and Nagi's team, already causing quite a stir with his conflicts with Nagi. Isagi is trying to see a way to mediate these two, which will be very complicated.

The first selection is the round-robin tournament of five teams of V, W, X, Y and Z in Building Five, with only top two teams being eligible to move forward. Isagi's Team Z, the lowest ranking team, plays against Team X. But because of the special rule that allows the top scorer of the team to proceed to the next section, the match turns into chaotic scrambling for the ball, regardless of the teams. It was Barou Shoei, Team X's formidable striker, who changes the flow of the game.

The Z team has only one game left to play, and it will decide the future of its members. While everyone seems to be nervous about the kickoff, Isagi is trying to find a way to score goals without anyone's help.

When Itoshi Sae scores the first goal of the match, the crowd goes wild, making it clear to Team Blue Lock that they're here to watch Sae. Rin changes the strategy and Nagi makes the memorable first goal for Team Blue Lock.

Shidou joins the match in the second half and throws everyone off with his crazy play. After he scores a free kick, Chigiri and Niko get swapped out with Reo and Hiori. With Blue Lock’s new formation, Reo proves his worth with Chameleon Defense.

Shidou enters the "flow" and unleashes his Big Bang Drive to score another goal. Isagi demands that Ego let them win, and Ego subs Barou in to cause chaos on the field. And the other players start to find their "flow"...

As Aiku looks back on why he converted to a defender, the U-20s uses their specialty quick attack. Reo copies Nagi's skill to hold them off. Isagi yields the last step to Rin, which is stolen by Barou who has waited for the chance in the dark.

Team Z was crushed by Team X, who centered the offensive play around egoistical yet skilled Barou. Responding to Ego's urge to "take up a weapon," the players discuss what their weapons are in preparation for the next match. However, Isagi struggles to identify what it is. Using "the smell of the goal" he felt in the game against Team X as a hint, Isagi and the others go against Team Y in the next match.

With many dropouts, the "Blue Lock" project enters the second selection. A harsh second selection where former friends become rivals and former enemies become allies at a dizzying pace. There, Isagi meets Rin, the most powerful egoist ever, the younger brother of the genius Itoshi Sae, and the new Blue Lock ranking TOP ranker. Seeking further evolution, Isagi's fight advances to a new stage.

Isagi and his friends barely manage to win as Barou awakens as the "villain" of the field. In order to cause a further "chemical reaction", they take away the only player who showed their ego to "eat" themselves and advance to the force stage. On the other hand, Bachira, who is looking for an opponent for the next match with TOP3, sees through the existence of a "Monster" in himself during training with Rin, and faces his own "ego".

In this new stage of the second selection, the white team formed by Isagi, Nagi and Bachira will finally go against the top three in the ranking of the previous stage, running the risk of losing one of their three players if they lose the game. Still, the willingness to test their new skills against the top three is much stronger in the white team members. Will they be able to win this match?

After the match with Team Y, Isagi's rank moved up, and he became the center of the team. The next match is against Team W, led by the Wanima twins. Their eye contact and impeccable combinations are hard to beat. They used to play in the same team as Chigiri Hyoma, and they use his past trauma to egg him on. Chigiri says that he joined Blue Lock to find a reason to quit soccer. Team Z leads the game with Kuon's play. Everyone's elated for the good start of the match, but Isagi feels something is off.

Bachira sheds his ideal of the "monster" and finally awakens his own ego. Until now, Bachira had been seeking someone to follow, but now he charges forward towards his own goal, finding in Isagi a companion who trusts in his ego. Isagi performs a decisive cut, but the ball made its merciless way to the victor's side... The time for the ultimate showdown has arrived, making sparks fly as the eight egoists clash. Which team will emerge victorious in the second selection's "Rivalry Battle"?

Rin chooses to add Isagi to his team, and together with Bachira they pass the second selection. Waiting for them on the other side is a 5-on-5 match against a team of world-class players. Thanks to Isagi, Bachira, and Rin's coordinated play, they manage to score the first goal, but they soon find themselves overwhelmed by the opposing team's superior skill and ability. Despite this, they desperately keep fighting until the last second, unleashing their own "egos." As a result, the "Blue Lock" project, aiming to produce the world's greatest striker, enters a new phase.

Isagi realizes that the conclusion of the match is in his and Naruhaya's hands. However, how will he go about beating Naruhaya? This will be a game that will not depend on genius players, but on "mediocre" players.

Isagi captures everything that’s happening in the field to see its future, and Nagi controls the space. Their combination bursts, and their series of play lights up the fire in Rin. Rin’s dominating play that encompasses everything on the field, from the skills of all players, the ball, positioning and tactics gradually corners Isagi’s team once again. As chaos and ego clash, one man is covertly sharpening his fangs to devour the game dominated by Rin.