Despite Ojo's overwhelming lead, the Devil Bats keep going. Unfortunately, for Ojo, Sakuraba strays onto the field and gets knocked down by EyeShield. Sena scores a small victory for himself, having managed to finally out-run Shin.

Sena may be on the Deimon Devilbats football team, but he realizes that he doesn't know anything about the game of Football. Can Sena learn how to play, or will he give up before the Spring season?

Chameleon Habashira Rui gives Sena a lift to the stadium on his motorcycle. In the game, Amino decides to target Monta and tackle him on offense and defense. Amino scores another touchdown and Ishimaru and Monta both score touchdowns for Deimon. The current score is 14:12. Sena is launched off the bike and lands in the stadium with a flashy appearance. He reveals his Devil Bat Ghost, a technique where a runner increases his speed and turns so that the opponent cannot predict where the runner will turn, while the linebacker sees a ghostly image where the player seems to have disappeared like smoke.

While searching for new players, including a Reciever, Sena finds a player named Raimon Tarou, who has great catching skills. But he is too late because Raimon is already trying out for the baseball team.

The Deimon Devil Bats have returned home. Hiruma must decide on the regular players. Everyone but Yukimitsu is chosen. Yukimitsu will continue to be a bench player.

Realizing that Habashira's not nearly as scary as Shin can be, Sena leaves him and the Zokugaku Chameleons in his dust. Deimon wins 48-28

Its Sena's first game as EyeShield 21, and he doesn't know what to do. As the Deimon Devilbats lose, Sena also has to make Mamori believe he isn't EyeShield 21, or he won't be able to play for the Deimon Devilbats ever again. Can Sena get the winning touchdown and keep Mamori beliving he's the team manager and not EyeShield 21?

Realizing that Sena's stamina for running is under-developed, Hiruma developes an amusing way to help him work on it. Meanwhile, Mamori begins to suspect that Sena is really Eyeshield-21 and sets out to prove it.

Cerberus gets switiched with a girl's doll, which has a diamond hidden in it by two wanted criminals.

The Devil Bats are resting at the Wild Gunmen ranch. The coach has agreed to train them. Hiruma mentions that Deimon does not allow extra-curricular activities after the third year so it's his and Kurita's last chance for the Christmas Bowl. That means they must improve in 40 days, not 405 (a year plus 40). Hiruma says the team must do the death march and that he will complete it even if it kills him. On the day the Devil Bats are supposed to return to Japan, the whole team chooses to go through the death march along with Hiruma and Kurita.

Deimon are up against Amino, a school where they use medical science to increase their muscle sizes. They are all artificial muscles. Sena follows "idiot" Taki onto the wrong bus and ends up to a faraway place, Nagano. Meanwhile Deimon, without their ace, must defeat Amino. At first the Deimon defensive linemen cannot hold Amino back and have a touchdown scored on them. Then Doburoku brings in a truck and tells them to push it until they remember how to push with their hips instead of just their arms, just like during the Death March. Deimon pushes through the line and Monta catches a ball for a first down.

Monta and Sena are reluctant to recruit the legendary kicker "60 yard Magnum" Musashi back onto the team. Being misled by soccer students who say a person called "MUro SAtoSHI" is Musashi, Monta and Sena try to recruit him into the American Football team. Meanwhile, a player called Sasaki Koutarou, the Bando Spiders' famed "smart" kicker, appears to challenge Musashi. Will Sena and Monta be able to convince Musashi to rejoin the team?

Hiruma forbids Sena to use the Devil Bat Ghost until a real game in order to keep spies from getting looks at it. Meanwhile Shin and Ootawara from Oujou have begun practicing in a corporation league, the Shuuei Elephants, to better hone their skills. The freshmen of the Devil Bats have decided to go to the same stadium, Shuuei Stadium, to inspect the next playing field. The Shuuei Elephants and the Samurai Warriors have an important practice match together, but Taki Natsuhiko leads some of the Samurai Warriors' regulars onto the wrong bus to Nagano Hot Springs (again). Due to the Samurai Warriors' lack of players in the match, Komusubi and Monta substitute and play in the corporation league practice match. Sena is determined to play in the match as well but cannot play due to the lack of an eyeshield, but Suzuna manages to find an eyeshield so Sena saves the match when he enters the game. But Shin wants to see Sena's devil-like Ghost Run instead of it being sealed.

Deimon's game against the Guts begins. Hiruma tries to put pressure on them with words about this being their last chance to go to the Christmas Bowl. However, the Guts' coach has replaced the regular players with athletes from other clubs in order to win, and the regulars aren't allowed to play. The Guts intercept the ball near the beginning of the game and score a touchdown and get the extra point, but after seeing them play, Hiruma lowers the Guts' previous 1% chance of winning to 0%.

It is Sena's first day of High School. And from now on his school life will be different. Sena has vowed not to be made a gopher like he has been in the past. But its bad luck for Sena after the Football Team Captain, Hiruma catches Sena running away from the Hah Brothers. Will Sena join the team and help the football team make it to the Christmas Bowl?

While running some errands for Hiruma, Sena runs into Shin, who team up to stop two theives trying to steal from the Devil Bats. In the end, the two theives turn out to be from the Zokugaku Chameleons.

Hiruma tries yet another insane method of training the team, while Monta learns the importance of passing routes when the team sees Ojo nearly defeated in the spring tournament final.

The game between the Deimon Devil Bats and the Taiyou Sphinx continues. The Devil Bats are gaining points as they begin to outsmart the Sphinx's famous Pyramid Line. The first half ends, and Deimon is only one point behind in the score. Monta is disappointed in himself after his performance during the first half, so he vows that he will ""restore his dishonor"" and make a super max catch! However, when the Sphinx decide to put in their cornerback, Kamaguruma, Monta will feel the full force of Kamaguruma's ""chariot bump!""

Monta is having a difficult time trying to get past the Spinxs new weapon againt them. Everytime Monta goes to catch the football, he is met with a BUMP. Monta doesn't give up in his attempt to get the ball. With the support of his team Monta is able to overcome this obstacle and get past the enemy. With this Monta scores a Touchdown putting the Devil Bats back in the game with only 35 seconds to go. Hiruma gives the order for Sena to ram the opponent or does he.

The Deimon Devil Bats are playing against the Nasa Aliens. The Nasa Aliens get a jump in the game against the Devil Bats with their very long pass the Shuttle Pass.

The Devil Bats truck gets stuck in a pothole; with one final push together as a team, Deimon overcomes the last obstacle. Arriving in Las Vegas, on the pirate boat outside Treasure Island, Doburoku confronts Sena to see if the ghost move is complete. Sena passes through Doburoku with the new Devil Bat ghost move, and Doburoku realizes Sena's unlimited potential. At the hotel, everyone rests in preparation for the journey home. The next day, Hiruma sells Doburoku's truck and tells him to go to the casino and bet his all on roulette 21#'s on red 21. The money pays off Doburoku's debt and he returns to Japan with the team.

Taki must pass the entrance exam to be able to play for Deimon. Suzuna tells them Taki has failed every single school with American football in Japan (except Deimon which has accepted every student due to their small number of application students, but Natsuhiko left Japan before being accepted). It turns out Taki is indeed an idiot and Suzana leaves in frustration, with Sena following to comfort her. As it turns out, Taki can answer the question if it is somehow related to American Football. Taki does the exam; a bare pass is 200. At night as the marker is marking the sheet when Hiruma's shadow appears behind him. As the marker announces Taki is safe with 203 points, Hiruma's shadow disappears, and the marker also mentions how he feels that he is the one who "made it." Deimon beats the Zokugaku Chameleons in a practice match because of Taki. At the end, the Zokugaku Chameleons are seen carrying their motorcycles on their backs home as their punishment from their manager, Megu Tsuyumine.

It's day 3 of the Death March, and Toganou and Kuroki decide to quit and run away. Juumonji tries to convince them to come back.

Sena applies all the training from the death march in the match. When he scores the winning goal, Sena's last opponent declares that he saw a ghost beside Sena as he ran. When the names of those who passed are read out, Suzuna's brother's name is skipped. He didn't make it, and so Sena asks him to become a member of the Deimon Devil Bats. He accepts, and the Taki siblings and Sena leave. Right after that, the announcer says Sena's name...

Now substituting in a corporation league game, Sena and Shin finally get the rematch they've been waiting so long for. However, Shin is disappointed as Sena refuses to use the Devil Bat Ghost due to his previous orders from Hiruma. The Samurais are losing due to Ootawara and Shin's entrance into the game, and the line cannot withstand Ootawara. The Huh-Huh brothers arrive and see Sena, Monta, and Komusubi playing, so they too join the game. Shin tells Sena that since he finally got the chance to face him, he won't hold back, and that Sena shouldn't either. With the encouragement of Monta, Komusubi, and the Huh-Huh brothers, Sena decides to use the Devil Bat Ghost.