Eyeshield 21 enters the Chameleons' locker room and cuts up all their uniforms in the middle of the night. Sena said he did not do it. The Chameleons enter Deimon and try and find Eyeshield 21. They find out the Scorpions were impersonating Eyeshield.

Chamelon and Poseidon start their game. Poseidon has a great advantage because they have tall players. On the other hand the Chameleon team is disunited. Habashira's team gave up halfway through. Habashira tries to encourage them and force his team to win, but fails. In the end Rui confronts Hiruma and cries out through his tears, "WHAT WAS THE DIFFERENCE BETWEEN YOU AND ME!"

The scorpion is playing really dirty. They are also predicting the Devil Bats' every move. Hiruma disappears during the game. Kurita must stay strong to win against those cowards.

At the end of the first quarter Mamori opens the game plan Hiruma gave her, which shows that Hiruma predicted every single thing that happened in the first quarter. At last Hiruma appears. The Devil Bats win the game with the help of Hiruma's Devil plans. Hiruma confirms his new slave after the game.

After being told by Mizumachi from the Poseidons that he is no good at football, Komosubi runs away from him. He thinks that because he is short, he is useless to the Devil Bats. Everyone goes looking for him and they convince him to return home.

Komusubi starts learning sumo. With his special training, he finds a way to overcome the physical difference between himself and Mizumachi and Kakei.

When Monta and Sena are sent to spy on the Poseidons' final practice before the game, they are caught, and Kakei tells Sena that he wants to challenge Eyeshield 21 from the Devil Bats and prove to him that he will never match up to the "real" Eyeshield 21. Sena gets past him using the Devil Bat Ghost, but Hiruma, who had been watching, tells Sena that it was not Kakei's true strength he was running against.

The game against the Kyoshin Poseidons is about to begin. Kakei tells Sena that he'll prove once and for all that he's not qualified to bear the name "Eyeshield 21." Hiruma then states that the taller team won't win, but the team that wins will be the team that is better at American Football.

The hidden ace of the Deimon Devil bats appears, being the crucial key to their new formation: Wishbone.

The Devil Bats have made a comeback, the score is 14-12, and Deimon has the ball. Kakei finds a way to exploit the weakness of the wishbone formation. However, Hiruma finds another way to overcome their new plan, and they score another touchdown, putting the Devil Bats in the lead.

The game continues with a stalemate with both teams trying to break the trend and score, knowing that this will lean the game highly in their favor.

The Poseidons reveal a new formation called High Wave. Hiruma immediately spots its weakness, but Sena cannot get past Kakei. The ball is turned over to Kyoshin, and they score a touchdown, giving them a five-point lead. They try to get an extra two points, but due to Hiruma's plan, the ball is intercepted by Monta. With 1 minute left in the game, Deimon's only hope is for Sena to get past Kakei.

Sena breaks through Kakei's Moby Dick Anchor, but he is stopped just inches from the touchdown by a desperate Mizumachi. Hiruma uses their last timeout with two seconds remaining and the Devil Bats behind by five points. They decide to have Sena jump over their line in a joint combination with Monta so that he goes twice as high. The plan succeeds, and they score a touchdown, winning the game. Kakei tells Sena that it no longer matters whether he is the original Eyeshield 21 or not, but that what matters is that he is one of the most excellent players Kakei has ever seen, and that Sena is Eyeshield 21.

Riku appears and talks about his past and how Sena could run because of him. Riku asks Sena who he thinks is the best player. His answer? Shin. He then asks why and Sena says because "his power and speed are incredible." Riku is part of Seibu who missed the game before due to his pride.

The whole Deimon team goes to a sports meeting where the team is divided into two teams, Mamori san, Hiruma, and Yukimitsu (the smart team) vs everyone else on the football team (the brawn team). They play tug-o-war and other sports. The "brawn team" finally finds out that Hiruma has plotted all the sports games to teach the team how to do the "bump" in his evil plotting ways by handcuffing everyone's hands (a trick first used by the Taiyo Sphinxes team).

Ishimaru misses practice because another member of his athletic club injures his knee at a practice and he can't run for three weeks. Sena offers to send Eyeshield 21 to run in his place but figuring Hiruma won't let him, goes as himself. Sena helps the athletic club reach the finals, where they win first place.

Riku learns that Sena's speed has increased, and also that he is secretly Eyeshield 21. Hiruma confronts Kid and tells him that between Riku and Eyeshield 21, Eyeshield is the fastest running back. He asks who Kid thinks is the strongest quarterback in Kanto. His answer: Yoichi Hiruma.

The game against the Wild Gunmen begins. Doburoku tells the Devil Bats that if they lose concentration for even a second, they will surely lose. Deimon kicks off the ball first and it is received by Riku. He shows the power of his technique, the Rodeo Drive, by running straight to a touchdown. However, when Seibuu kicks off to Deimon, Sena returns the favor by doing the same, running a straight touchdown, being stopped by Riku just a second too late.

Unable to stop Kid's Quick and Fire technique, the Devil Bats decide to reveal the fruit of their bump training. Seibuu counters by bringing in the Iron Horse, Tetsuma. Monta is unable to stop him, and he scores a touchdown, with the extra point successful, making the score 14-6. Hiruma tries to trick Kid into a quick pass by using a Zone Blitz, but he fails and Kid rushes to a touchdown.

The Devil Bats are in desperate need of a kicker, and Musashi is watching the game from his father's hospital room. Without a score before halftime, the Devil Bats have a slim chance of victory, so Hiruma decides to kick the field goal himself. The kick is close, but it hits the side pole and is no good. Using a strategy that relies on nothing more than Kurita's brute force, the Devil Bats get a touchdown, lessening the point gap.

Trying to prove to Sena that he is the better player, Riku fumbles the ball. Sena intercepts it and runs straight to a touchdown. With Deimon only down by 2, Seibuu gives the ball to Riku. Sena tries to stop him, but Riku gets by him easily, scoring another touchdown for Seibuu.

There are 9 minutes left in the game, and Deimon is behind by 9. They try to make up the difference, but Sena cannot get past Riku. With only 1 minute left, Sena finally gets past Riku's Rodeo Drive and scores a touchdown. Deimon is only behind by 3 points.

Kid tells Hiruma that he fell directly into his trap, and that everything is always too good to be true. Hiruma reminds Kid that he told him that Eyeshield would definitely win, and Kid tells Hiruma not to forget that he said Hiruma Youichi is the best quarterback in the league. They part ways, saying that the rest of the battle will be a battle of wits, and Hiruma decides to do an onside kick.

After losing to the Seibu Gunmen, Tetsuma saves Monta from getting Deimon suspended from playing. Hiruma reveals there is a seat left in the Tokyo Tournament so they still have a chance to be in the Christmas Bowl. Deimon attends a summer festival. Mamori invites Riku. Riku tells Sena to promise to remove his eyeshield mask before the Kantou Tournament.

The Bando Spiders play against the Oujou White Knights. The Spiders are in the lead due to Kotarou's kick. Deimon is stunned that a small Bando player can take down Otawara and the team seems to know Oujou's passing routes. However, in the end, Oujou wins 14 to 3 and are set to play the Wild Gunmen.