While on a job observing stray cats at a shrine feeding themselves,the Yorozuya waiting for the boss cat to show up with the name Hoichi. But as he finally shows up, and Kagura and Shinpachi jump out of the bushes to capture him, he escapes easily. So they ask Gintoki to help out, when they notice that he mysteriously disappeared.

Okita is going through a selection of sweets at the cake shop. As Okita leaves the store, a girl suddenly comes up and stabs him!! Calling Okita her father’s killer, the girl’s action seems to be fueled by a past case called the “Rokkaku Incident.” What is the connection between this incident and Okita, as well as the girl’s father? And more importantly, what’s the fate of Okita who’s been stabbed right at the start of this episode?!

Part 1 - While researching the Anti-Foreigner War, a reporter interviews Nagai, a former reporter from the war called Nagai in order to know about the White Demon's (Gintoki's alter-ego) activities in such time. He chronicles the events from war, explaining how the samurais decided to fight against the aliens. As Nagai is about to talk about the White Demon, he replaces him with the fictional robot Gundam to celebrate the 30th anniversary of the franchise with the same name. Part 2 - Kagura starts going to Kabuki-cho's physical fitness lessons where she meets a kid named Hongou Hiashi, whom she befriends. Suffering from a dangerous disease that weakens his body, Hiashi is absent from various lessons even though he promised Kagura to go to every lesson. During a rainy day, Kagura is the only person to come to the lesson, but Gintoki and her friends decide to join her. Next day, Hiashi returns to another lesson and sees Kagura once again.

While protecting Gintoki from the stray cats, Hoichi and the cats get caught. Soon Gintoki, Katsura and Kondo goes to save him.

Consumed by negative emotions, Douman is transformed into a giant demon. He now possesses within his body Antenmaru, a demon sealed away over a millennium ago by the desperate efforts of the Ketsuno and Shirino clans. As Seimei is about to be killed by Antenmaru, Gintoki stands before him with his aura-charged wooden sword. Gintoki charges his sword with everybody's energy including Douman's in order to overwhelm Antenmaru and bring a sunny day. After Antenmaru is destroyed, Gintoki leaves so that Ketsuno Ana will not know about the fight. However, he requests Gedomaru to make Ketsuno Ana to give him her autograph. Douman appears alive and ends up having a childish fight against Seimei. As Odd Jobs leave, Ketsuno Ana expresses her thanks to Gintoki in her weather forecast.

It's Christmas in Edo and Kagura writes her father a letter. She is sad that Santa Claus never visited her. Moved, her father Umi Bozu dresses himself up as Santa and hides watching Kagura, Otae, Shinpachi and Hasegawa celebrate Christmas. When he finally jumps out of the bushes to surprise her there is another Santa, a dressed up Gintoki. Both of them think that their counterpart is real. They start punching each other when another three Santas show up (Sachan, Kondo and Kyubei). Kagura decides on a contest to see who's the real Santa.

Gintoki and Shinpachi start re-investigating the Rokkaku incident. Upon learning what really happened from Kamiyama, one of Okita’s subordinates, they leave to save Okita. Meanwhile, Okita is captured by Sotatsu Tendo after falling into his trap involving Kirie. Tendo babbles on about how he returned to seek revenge against Okita, who destroyed the Sokai Faction, and goes on to reveal the truth about Kirie’s father.

Students at a temple school share their independent research projects completed during the past summer vacation. After one of the students finishes reading a well-written journal, the last one to present is a kid named Daigoro. Sleeping in on the first day of summer vacation, Daigoro arrived late at the radio exercises and met Madao, and decided to keep an observation journal about him.

Having turned into cats, Gintoki,Katsura and Kondo (who turned into a gorilla) are brought by Hoichi to Otose to get food. While eating, Otose tells them that if caught, they can never return to this town.

Seimei takes the risk of being dismissed as lead guardian of Edo by undoing the Shikigami placed throughout the city, and fueling himself with all their power to face Douman. Their fierce magic clashes, and Douman is forced onto the defensive as Seimei's techniques overwhelm him. Just when the battle seems over, Douman recalls the history between his family and the Ketsuno clan as repeatedly told by his father.

The Odd Jobs are requested to remove a hornets’ nest on the temple grounds. Fully armored in protective suits, what awaits the trio is a ginormous hive, like none they’ve ever seen before. Arguing that this wasn’t what they signed up for, Gintoki prepares to leave, but Kagura goes off and destroys the hive. The trio quickly plays dead to avoid getting attacked by the raging hornets, but are instead greeted by something quite unexpected...

After eating egg over rice for three days straight, Gintama is fed up with Kagura being the cook in charge and doesn't want any egg over rice. Frustrated by Gin's reaction Kagura goes over to Shinachi's house to talk to Otae. Then Otae asks her if she wants to join a cooking class with her. Together with Shinpachi and other odd faces they start the cooking class.

The episode begins with a short sequence about Kamui transferring into Yato High School and is told from Abuto's point of view. While the Shinsengumi are out on a mission, Hijikata notices snot coming out of Kondo's nose so he wonders how he could tell him without the others noticing Kondos problem. In the end the snot even takes control over Kondo's body and it comes out that Kondo's true identity is being an Amanto in snot form. As Hijikata destroys it, he realizes it has been all a dream.

After learning from Gengai that Tama has been infected by a computer virus, the Odd Jobs trio launch “Operation Midget” and enter Tama’s body to eradicate the virus. Inside, they find an injured man dressed in a white body suit who begs them to deliver a message to the king. Arriving at the Leukocyte Kingdom, the gang finds the place filled with RPG-themed shops such as weapon stores, inns, and casinos. The group decides to enjoy this RPG-like world by playing at the casino and visiting the church to save their game. Joined by Tama’s avatar, Gintoki and the gang head out in search of the Leukocyte King. Let the adventure to destroy the computer virus begin!

Character popularity results are announced to celebrate Gintama’s 182nd episode! Shinpachi is alone at the café, feeling depressed after learning that he’s still in 8th place in popularity, unchanged from the previous ranking. Yamazaki, who is in 9th place, drops by to say that boring characters such as they should aim to become the No. 1 steppingstone for others. For Shinpachi and Yamazaki, 8th and 9th place should hold the same meaning as 1st. But in reality Yamazaki can’t give up the hope of ranking higher, and runs off in tears. Shinpachi can do nothing but watch, when suddenly Yamazaki’s scream is heard in the distance!

Lately the weather forecasts Ketsuno Ana is predicting are always wrong. When Gintoki meets her on the streets he takes her to the Yorozuya's place to help her.But after a monster attacks the house,it is revealed that Ketsuno Anna is an onmyouji and some other onmyouji is targeting her. After that Gintoki and the others are on the way to the Ketsuno residence,where they fight some odd creatures.

In order to save Ketsuno Ana, Seimei agrees to Douman's proposal of a Shikigami Tag-Team Mystic Death Match. Seimei arrives at the arena only to be captured by Douman, but Gintoki and the gang appears to lend a helping hand. The first match ends in a draw after both the Ketsuno and Shirino clan contestants are disqualified. After seeing how they performed in the first round, Seimei accepts Gintoki and the gang's skills, and together they prepare for the next round. Rather than making a casting spells fight, Gintoki and Ketsuno Ana's shikigami, Gedomaru, try to beat up Douman, but are caught in one of Douman's illusion and Gintoki has his testicles rip off by Gedomaru's weapon. As Gintoki's testicles are accidentally damaged by Gedomaru, Seimei decides to fight Douman alone while the shikigami heal Gintoki.

The fierce battle begins over the title of the Official Tsu Terakado fan club. Tosshi, Hijikata’s loser otaku personality created from a demonic sword, was once thought to have been defeated by Hijikata’s strong will, but in reality the personality still survives within him. By reigning as the king of otaku, Hijikata plans to put Tosshi’s soul to rest. Tosshi’s road to the king of otaku versus Shinpachi’s love for Otsu-chan. Who shall be victorious?!

Hijikata is on a stakeout near the apartment’s dumpster. There, a sleepy Gintoki comes to throw away his trash. Finding Gintoki’s persistent intrusion a burden, Hijikata arrests Gintoki for insulting and obstructing an officer on duty. But while pushing and shoving each other, Hijikata accidentally handcuffs himself to Gintoki. Hijikata calls Okita who is staking out a different location to come and unlock them and take Gintoki to the Shinsengumi Headquarters. Instead, Okita puts a second pair of handcuffs on Gintoki and Hijikata. In the midst of all this, the Radical Joi Faction makes a move.

Weather girl Ketsuno Ana is giving the wrong weather forecast. Turns out she comes from a line of traditional Onmyojis, and her forecast is not derived from mere fortune-telling but actually by using her Onmyoji powers. But her weather forecast is being sabotaged by someone who is unhappy with Ketsuno Ana's career as a weather girl despite her being an Onmyoji. Gintoki and the gang arrive at the Onmyoji mansion where they come face to face with Seimei, Ketsuno Ana's older brother.

The city is hit by a bad flu one winter day. Even Otae is sick with the flu. Shinpachi gets some long-awaited time alone with his sister and is tending to her when he gets a visit from Gintoki and Kagura. Turns out the two have also caught the flu and want Shinpachi to look after them. Irritated by Gintoki and Kagura’s abrupt visit, not to mention their selfish demands, Shinpachi is surprised when even more sick guests arrive.

Gintoki strikes down Jiraia and frees Tsukuyo from his twisted web. Chiding Tsukuyo for trying to shoulder every burden herself, Gintoki goes on to say that rather than throw away your life to die beautifully, it’s far better to live and be yourself – even if your life is not a pretty one. And finally, Gintoki expresses his anger toward Jiraia for betraying his own student, despite calling himself a master.

The city of Yoshiwara is making its recovery, though Tsukuyo has shut herself in her room. Hinowa asks Gintoki to help Tsukuyo have some fun for a change. It turns out that Tsukuyo is only pretending to be sleeping in her room, when in reality she is sneaking out to perform her routine patrol. The gang decides to come up with a plan to help Tsukuyo cheer up, even while working.

Tosshi finishes the preliminary race in first place by rounding up all the contestants to block Shinpachi’s team from making the goal. Despite Toshi’s scheming, team Otsu-chan fan club makes a surprising comeback to finish in second place! Hijikata points out that the team cheated by changing their member lineup, but with his own member suspected of using a taxi, the objection is dropped. Kondo and Kagura also finish the race after taking the wrong route and being dropped off by the police. Meanwhile, what happened to Shinpachi who’s still in the race? That being said and done, the main event finally takes place!

Gintoki and the gang arrive at Tama’s main system. The group tries to devise a battle strategy for fighting the Great Archfiend Tapir’s black body suit minions. But they can’t come to an agreement. Deciding it’s a waste of time, Kagura charges alone into the swarm of black body suits. Using this as a diversion, the group storms the enemy base, only to be surrounded after Gintoki and the Leukocyte King get into an argument. But during their rumble to decide who’s the strongest, the two wind up destroying the black body suit minions. And finally, the Great Archfiend Tapir appears!