Golgo 13 is hired out by a mobster to go to Massachusetts and assassinate an anti-crime analyst during the state's presidential elections. When Golgo 13 is arrested by FBI agents for assaulting an agent, he is grilled regarding the murder and the PPS-made revolver that he used until a lawyer named Mr. Barton begins to question the loopholes in the FBI's accusations against Duke.

Golgo 13 is arrested and sent to Pandora Island Prison, an isolated prison somewhere near the Alaska mainland to rescue a prisoner named Patrick Zalas despite being under the thumb of a sadistic and corrupt warden named Kickers. But after the two escape, Golgo executes him under orders of Patrick's bosses.

Brigitta, a female assassin is hired to kill Golgo 13 but she knows she is no match for him. As a means to even out the contest she meets with him and gives him the job of taking out a mafia boss who is using a meat factory as a front. Whilst Duke is preparing for the job she informs the boss of Golgo 13 who promptly sends over his band of goons to kill him. However he escapes and leads the goons straight to the meat factory and lures them out of their cars where the sirocco wind is blowing the sand from the desert restricting their visibility, where he picks them off. Brigitta planned this as a way to put Duke at a disadvantage so that his eyesight would be impaired when it came to the duel. Donning a pair of goggles she sets out to finish off Golgo but she underestimates just how well-prepared he is.

Victor Lance, a paparazzo photographer, decides to take a photo of Golgo 13 before he retires for good. Unfortunately, Golgo 13 takes out Lance's camera with the help of reflected sunlight on his camera before Golgo takes out his target in Britain.

A woman, whose husband cheated on her, feels she wants to die to escape the pain he caused. By chance she shares a taxi to the airport with Golgo 13 and also the seat next to his. When the plane is forced to perform a belly landing Golgo reveals to her that she does actually want to live and enjoy life. At their destination, having made love at night, she follows him to his job. Golgo can't allow a witness to the job and so kills her.

Following a successful assassination, Golgo's escape plane crash lands on an island where the American military are testing the new SDR2 armoured suit against captured terrorists. The inexperienced pilot is ordered to kill Golgo as he is a witness to the new technology. As the pilot becomes increasingly unstable after being outsmarted several times Golgo is finally able to destroy the SDR2 with a well-placed shot. The American officer has Golgo brought in where he demands his services as an advisor. Not wanting to comply Golgo kills the officer and the guards.

A real-estate businessman, Johnson, discovers that a bet he lost years ago to Crump, another real-estate business man, was rigged and resulted in Crump becoming very rich and powerful. In a bid to exact revenge he hires Golgo 13 to kill Crump, who has a love of the theater, but not anyone else. As Crump is constantly surrounded by bodyguards, Duke has to use a specially made disposable gun and time the shooting with the sound effects of the play and hide the gun before anybody notices.

Loi, an Asian film producer is looking to shoot on location in America but the actors and producers are continually being assassinated. In order to protect the star of his movie, Lee, and secure the future of the Kong Kong film industry, he hires Golgo 13 to kill those responsible for the attempts on Lee's life, however he does not know who is being the attacks. An American company is behind the attacks using the DIA to try to kill Lee out of fear that the Kong Kong film industry will bankrupt them and erode into the American film industry, reducing the American influence around the world. Golgo moves Loi and Lee to a Chinese secret armored building and when DIA elite troops attack, he has planned its defense and is able to kill most of the troops and determine who is responsible. Some make it to where Loi and Lee are and fatally shoot Loi. Golgo shoot the halogen gas fire system releasing the gas into the airtight room. Shooting the window with a grenade the air pressure difference sucks out the remaining troops. Golgo finishes the assignment by killing the one responsible for the assassinations.

Albert Stantz, a governor election candidate, hires Golgo 13 to assassinate Wassant, a philanthropist dedicated to transforming orphans into valuable members of society. They both shared a history belonging to an anarchist street gang and Wassant is now trying to atone for his past. However Duke is involved in a car accident where his dominant right arm is severely injured and next to useless. He finds Dr. Cutter surgeon with amazing dexterity and skill to heal his arm. Overhearing a telephone conversation between Cutter and another orphan, he learns that Cutter is one of Wassant's orphans and deduces that Stantz had mentioned Golgo 13, breaking Duke's terms of agreement. After successfully shooting Wassant and leaving the weapon at the scene he shoots Stantz, again leaving the weapon at the scene. The M16 used to kill Wassant was modified to be shot left-handed while the other M16 was unmodified. Cutter theorises that Duke had used his left hand as an obligation to him for healing his arm. The series ends with Duke leaving for the airport.

Golgo 13 assassinates a drug lord after he attacks and wipes out his FBI and NYPD escorts before heading to Hong Kong to do another job when he finds out that a lookalike was responsible for hiring him. After realizing that the lookalike was merely a gangster named Spike Jordan, Duke kills him with cyanide gas.

Golgo 13 is hired to assassinate the Jordanian emissary to the United States, while the emissary transfers from a plane to his helicopter. However, a young, hot-shot sniper is hired by the American State Department to take Golgo out before he accomplishes this. By leaving two disassembled rifles in fake sniping points, Duke is able to fool the inexperienced gunman. Golgo then uses an M-16 to take out both his new rival and the helicopter.

Golgo is deployed on a covert mission in England to locate and kill a reclusive millionaire named Vargas Walton who has been "keeping" women as his guests in his estate located at an artificial island, ready to have healty organs from them to be harvested and kept ready for donors while using the funds from organ donations to finance several terrorist groups. The millionaire is protected by high-tech bulletproof glass, making an assassination attempt from the sea or air nearly impossible coupled with an ex-expert Olympic shooter named J.J. as the head of his bodyguard detail.

A man hires Golgo 13 to kill Carronte, a terrorist in a maximum security prison, to avenge the death of his family in a random act of terrorism. However Carronte's cell is behind a revolving door and sliding door and only has a small aperture window. Golgo 13 has to maintain his posture for ten hours in order to make the shot. Knowing this is impossible he contacts a man who was involved in an athletic doping scandal who can make a drug to relax his muscles. Golgo manages to shoot the target but his muscles are in a bad shape and requires professional massage to recover. The chemist had offered to do this but took Golgo's vital statistics to an FSB contact who would give him a huge reward for killing Golgo 13. As Golgo can hardly move he moves to kill him but does not anticipate how well prepared the professional is.

Golgo 13 is hired by a representative of the U.S. Securities and Exchange Commission when he was told to target a mafia financier in New York. Despite getting into trouble when his used .30-06 Springfield cartridge fell down from his apartment balcony due to the appearance of a stray cat, Togo tries to cover his tracks when a desperate detective of the NYPD starts to hound him over his stay in New York City.

Bob Stigner, vice-president of Conan Associates, hires an ex-Polish olympic athlete and soldier named Ledell Nikolavitch to assassinate the company's president and current leader Ted Conan in order to take over the entire company despite what Ted had done to help Bob escape from his past problems. However, Ted is wise to Bob's game and decides to hire Golgo 13 to assassinate both Bob and Ledell for good.

Golgo 13 is hired by Simpson a famed violinist from London Symphony Orchestra to sabotage a charity concert lead by his replacement Kaspersky by shooting the G-string from his violin.

In the aftermath of the murder of a Russian diplomat and his mistress, a woman walks into bar in a rough area where Golgo 13 is visiting. When he leaves she runs after him and asks him to buy her. Suddenly some men try to kill them but Golgo dispatches them. They go back to a hotel and have sex and again more men try to kill them. A chase ensues and they escape to the sewers where she reveals that she is a CIA assassin behind the diplomat's death and that the men are chasing her and that she managed to hire Golgo 13 for free. After killing all the chasing men she pulls a gun on Golgo but he is quicker and kills her first.

What began as a simple mission to stir up trouble for racers driving Japanese-made vehicles in a rally race turns into a match of sniping when Golgo 13 is targeted by the Savine brothers, ex-French Foreign Legion soldiers turned mercenaries armed with modified XM29 OICW and FN F2000 bullup assault rifles while being aided by customized digital scopes made by a European arms dealer named Kaizer in order to market them to numerous military forces from around the globe.

Golgo 13 is hired by the New York Mafia to assassinate an international assassin. She turns out to be Eva Kruger, a woman Golgo had met on a cruise ship when an unknown terrorist had planted a bomb on it. The two parted ways after he defused it to save the passengers despite being told by the ship's crew to evacuate with the rest of the passengers. But even though Eva decides to retire as an assassin and be a civilian, Golgo had successfully assassinated her from a distance on another boat.

Golgo 13 is hired by a MI5 operative, who had been able to infiltrate covertly the security detail of Louis Steinbeck III, an aristocratic man supposedly of French royal descent under orders from the British government. Golgo is hired to assassinate Steinbeck III as he is the leader of a secret rogue intelligence organization that sells out all types of classified information to various countries called MC-130. However, the MI5 operative/Steinbeck III bodyguard learns that Golgo did not target Steinbeck III but instead, went for the butler as he was the real leader of MC-130 when the hit was in operation.

Golgo 13 arrives at a hotel for Christmas but immediately falls under the suspicion of Dahl, the hotel detective that has heard about a recent spate of local crimes. When a known pickpocket tries his luck on Golgo he is caught and handed over to Dahl to deal with him. The pickpocket informs Dahl that he felt a gun on Golgo and so she decides to pay him a visit. She realizes that Golgo is not responsible for any of the local crimes but knows he's a professional there to do a job. Meanwhile Tommy Nabarro, a syndicate under-boss, is bailed. A policeman informs Dahl that Nabarro is staying in her hotel but he is to be assassinated as he knows about various crimes committed as well as a link between the syndicate and a senator. As Nabarro leaves the hotel on Christmas day Golgo causes him to turn around before sniping him from his room, so it appears that the shooter was at street level. Golgo checks out the hotel and walks past Dahl who is grateful that Nabarro was killed outside the hotel.

In Baltimore, Maryland, a rising American football star named Lionel Blue was assassinated by Golgo 13 by using his M-16 in a hidden rangefinger after being hired by a disgruntled football player via football gambler. An old-fashioned detective and the Baltimore police try to pin the evidence on him, the difference by the use of technology before Golgo 13 destroys the CSI Department's computer equipment with assistance from Flannagan despite being in a hospital under pretense of a potential cholera infection.

A small family-run motel hears news of a casino robbery when Golgo turns up to stay. Hank, the son of the family returns home with another man having executed the robbery. The blind mother is suspicious of Hank and is unnerved by Golgo, but the assassin leaves before anything happens. Confronting Hank, the mother accidentally shoots Hank and the accomplice escapes to deliver the money to his boss, without realising that Golgo has been hired by the mob to kill those responsible for robbing their casino.

Albert is from a proud family of French wine producers but his wines are being bought up by Yanagida, a Japanese man who cannot distinguish between vintages. After an auction where Albert fraudulently sells a 1900 vintage in place of a 1800 vintage, Yanagida hosts a party to show off the wines with several noteworthy sommeliers, who will be able to taste the fraudulent wine, attending. Desperate to retain his pride and not be exposed as a fraud, Albert hires Golgo 13 to shoot the bottle of fake wine during the uncorking. However at the party Yanagida invites Albert, as guest of honor, to perform the uncorking.

Linda, the wife of a mafia leader in New York City hires Golgo 13 for the purpose of assassinating him so as to start another life all over again in Florida. Things gets complicated when the mafia boss kills one of his henchmen after being accused of trying to be in an affair with Linda, resulting in a small gang war fight before Golgo 13 was able to get rid of his targets and unfortunately Linda when she decides to kill him.