Kenshin and the group are enjoying some tea time together. The group probes Kenshin to entertain them with an interesting story from his travels, specifically requesting "a story that's unusual, fun, and includes a villain's defeat." To answer to their demands, Kenshin decides to tell them about someone he met when he was visiting a foreign settlement in the lively city of Yokohama. A foreign doctor named Elder who was always wearing a mask.

Megumi is alone in Tokyo and coincidentally encounters Aoshi at Kamiya Dojo, who’s come back for another fight with Kenshin. Aoshi asks Megumi where Kenshin is, but she feigns ignorance. Unhappy with the lack of response, he threatens to kill her if she doesn’t tell him, when a man suddenly appears. Meanwhile, Kenshin encounters a young girl in the forests of the Hakone mountains as he makes his way to Kyoto.

The name of the girl that Kenshin encounters in the forests is Misao Makimachi, and she came from Kyoto to find Aoshi’s Oniwaban Group. Kenshin is surprised to hear his name. Upon seeing Kenshin’s reaction, she asks him if he knows where Aoshi is, but he keeps his mouth shut. However, Kenshin is touched by the emotions Misao has for Aoshi, in which he then decides to make the journey to Kyoto with her.

Kenshin steps into Shingetsu Village, a place where the Mishima brothers escaped from. The village is currently under the control of Senkaku, Shishio’s subordinate, who rules with an iron fist and uses violence to control the villagers. They are frightened of the violence and unable to do anything but obey. The village has decayed and exudes an unusual aura about it. Kenshin heads over to where Shishio is located with Hajime, who’s learned about the current situation and visited the village. In the background of these events, Eiji’s heart burns with the desire for revenge against Senkaku.

Sanosuke makes his way to Kyoto while trudging through the forest and getting lost at the same time. On the road there, he meets Anji Yukyuzan, a fallen monk currently in training. Sanosuke witnesses Anji unleash an astonishing strike up close, a technique that took Anji 10 years to acquire. There, Anji decides to teach Sanosuke “the Mastery of Two Layers,” an art of destruction. However, he requires Sanosuke to learn the technique within one month; if he fails, he will have to die. Against this unreasonable and excessive condition laid out to him, Sanosuke declares he’ll learn it within a week. The training for this technique, which will make or break his life, begins….

Kenshin and Misao arrive in Kyoto, where they’re welcomed by Okina, a grandfather figure to Misao. It turns out, Okina is a former member of the Oniwaban Group. Okina offers his help and Kenshin ask him to help find someone for him.

Kenshin obtains a new sakabato. Having involved Seiku in this conflict, he decides to leave Aoiya before inviting any further danger. Meanwhile, Misao stops by Shirobeko, a beef hot-pot restaurant where Kaoru and Yahiko work part time, where she learns Kaoru is searching for Kenshin.

Kenshin reunites with Kaoru and the rest at Seijuro Hiko's place, who is a master of Hiten Mitsurugi-ryu. Seijuro learns from Kaoru that Kenshin goes around the country acting as a rurouni who helps people, when he then decides to pass down the last ultimate technique of the Hiten Mitsurugi-ryu.

Aoshi injures Okina, leaves Misao behind, and shows up at Aoiya. He asks the Oniwaban Group where Kenshin is. Aoshi's in a ghastly state, but accepts what Yahiko and Kaoru tell him and decides to wait for Kenshin where Shishio is. Okina is on the verge of death but undergoes treatment by Megumi, who has come rushing to Kyoto.

As a result of passing down the ultimate technique, Seijuro endures a fatal wound.

Kenshin and Hajime realize that Shishio has another objective in mind that lies in Osaka. They meet up with Sanosuke there. To stop the Great Kyoto Fire, Kenshin asks for help from everyone at Aoiya via a letter while leaving Ryusaku Arato, the chief of police, in charge of supervising on the ground in Kyoto. Kenshin, Hajime, and Sanosuke head to Osaka Bay on a carriage after they finish their errands. Then, senior police officer, Hikozaemon Omi brings along Juro Kitazawa, a young 11-year-old boy who says he wants to meet with Arato.

Kenshin and Aoshi engage in a battle on the deck of the ironclad Rengoku. Behind the scenes, Sanosuke manages to board the ship and tries to blow up the engine room with the explosives he got from Tsunan. However, Shishio's underlings Hoji, Shuzo, Toshiro prevent him from going any further. Meanwhile in Kyoto, Arato faces off against Kamatari and Henya alone to buy time for the police forces to evacuate.

Shishio sends a dueling challenge directed at Kenshin, Hajime, and Sanosuke. The duel is to be held the next day on Mt. Hiei, northeast half way up the mountain in a small shrine with a six-storied torii. At Shishio's fortress, Hoji proposes a strategy to Shishio for an "absolute victory," when the Ten Swords assemble there.... And on the night before the duel, Kaoru talks to Kenshin as he stares up into the night sky.

Yahiko's heart is moved by Kenshin and he decides to quit pickpocketing. Yahiko refuses to pay Kanto Shueigumi and resists their demands, but he is beaten up. Kenshin comes to his rescue and settles the matter. Regretful that he could not solve the problem by himself, Yahiko wishes to become stronger. Seeing this, Kenshin takes Yahiko to the Kamiya Dojo.

Kaoru, Kenshin, and Yahiko visit Maekawa Dojo for a training session. The dojo is very lively, and Kaoru's training is well-received by the students of other sword styles. Then a swordsman named Isurugi Raijuta breaks in. He says he is concerned about the future of sword art, and the man, carrying only an actual sword, dismisses the idea of a bamboo sword match. In order to defeat the challenger, Maekawa, the master of Maekawa Dojo, takes on Raijuta, but...

Sanosuke is visiting the sukiyaki restaurant Akabeko, where he always leaves his bill on a tab. One of Akabeko's workers, Tae, asks him to go buy a Nishiki painting from the painting shop on her behalf. Though Sanosuke had no interest in paintings, he finds one at the store that catches his eye. It was a painting of the man he looked up to as his master back when he was a member of the Sekiho Army, Captain Sagara. With a gleam in his eyes, Sanosuke makes his way to meet with the artist of the painting.

Tsunan is seeking to clear his decade-old unresolved regrets surrounding the Sekiho Army. Empathizing with him, Sanosuke joins Tsunan in conspiring an attack on the Home Ministry. However, Kenshin was waiting for them inside the building to stop them. Seeing the former Revolutionary warrior standing in their way, the same way they faced off against Revolutionary warriors when they were in the Sekiho Army, Tsunan's fighting spirit burns. A flurry of explosives are hurled at Kenshin.

Raijuta, who wants to revive the murderous sword, considers Kenshin, who denies his idea, as an enemy. Raijuta initiates a fight using his actual sword, but Kenshin lands a blow that breaks Raijuta's sword and stops the situation from escalating further. After some time has passed, Yutaro, a boy who claims to be Raijuta's apprentice, arrives at the Kamiya Dojo. He claims he has come to challenge Yahiko, with whom he doesn't get along, to a match, but the way Yutaro holds his bamboo sword is no good...

The current Kenshin has left his past as a manslayer and wished for a peaceful world. Though Elder looked at him with eyes of confusion, Kenshin knew that Elder also shared this same desire for a healthy society and felt a sense of comradery with him. Meanwhile, another doctor called Deian Ishizu was scheming to get rid of Elder, as Elder did not seek monetary gain in exchange for her medical services, and Deian thought this was disrupting his business. Deian hires Espiral, a master of Western swordsmanship, to get rid of Elder. However, it seems Espiral has an entirely different motive for taking on the job...

11th year of the Meiji Era in downtown Tokyo. Kaoru Kamiya, the acting master of the “Kamiya Kasshin-ryu” style, was chasing after the “Hitokiri Battosai,” who was claiming to be a user of her sword style. One night, Kaoru meets a man carrying a sword. She immediately questions the man, but he seems to have absolutely no will to fight. “Is this the rumored Hitokiri Battosai?” As Kaoru dubiously looks on, the man introduces himself as Kenshin Himura, a “Rurouni.”

It's been a week since the resolution of the crossroad killing case, in which the name of the Kamiya Kasshin-ryu was deceptively used. Although the name of Kamiya Dojo has been cleared, there's no sign of an increase in the number of Kaoru's students. Then a passing boy, Yahiko Myojin, pickpockets Kenshin's wallet. Kenshin gives him the wallet, saying that it was his fault for allowing it to get stolen. Yahiko is repulsed at being treated like a child, but he has a reason to collect the money.

Yahiko, the sole student of the Kamiya Dojo, has started to step away from his training from time to time. He says he's merely going on a walk, but it is clear that he takes his swordsmanship training very seriously, and it doesn’t look like he's ditching to slack off. Curious about what he's doing, Kenshin, Kaoru, and Sanosuke decide to follow him. What exactly is Yahiko's reason for ditching?

Sanosuke's friend has died from an opium overdose. However, Kenshin and Sanosuke are perplexed at how he even got his hands on the drug, since opium is banned and too expensive for the average person to afford. Then a mysterious woman, Megumi Takani, appears before them. Kenshin and the others fight off Kanryu Takeda's private army who is trying to capture Megumi. However, Megumi is carrying opium...

The pursuers from the Oniwaban Group haven't made an appearance for over a week, and during this time Megumi familiarized herself with the Kamiya Dojo group. Sanosuke, however, was still unsettled and feeling frustrated knowing that Megumi was the one who made the opium that caused his friend's death. Then one day, Megumi suddenly disappears. In a letter they found from her, it stated that she had left of her own volition, but Kenshin and the others found this to be unnatural. They decide to head to Kanryu Takeda's residence, the man who was sending spies after her.

Kenshin, Kaoru, and Yahiko visit Akabeko, a beef hot-pot restaurant, where a group of democratic rights activists gets into a heated argument. One of them throws a bottle of sake, and it hits Kenshin, causing a commotion. Sanosuke Sagara, who happened to be in the store during the quarrel, stood up and blew away the activists with a single flick to the forehead. Seeing Kenshin's strength, Sanosuke's curiosity is roused and he asks, "You wanna buy a fight from me?"